Hypoxia/Hyperoxia controller : Biospherix

Biospherix Ltd. (USA)

Oxygen controller ProOx110
Oxygen controller create hypoxia and hyperoxia condition for various research experiment of cell, animal and plant. The equipment control oxygen level in chamber and incubator, which is semi-sealed. The oxygen sensor measuer the oxygen level in the chamber and incubator and it supply nitrogen gas for low oxygen and oxygen gas for high oxgen. The oxygen control range is between 0.1%-99.9%. The optional equipment we can offer make it reduce the gas consumption and come ture the application which is never before possible.

Oxygen and CO₂ controller poduct lineup
Application examples for cell culture
Application examples for animal and botanical experiment

Oxygen and CO₂ controller poduct lineup

Item	Feature
Oxygen controller ProOx 110	Control oxygen level in small chamber and incubator
Oxygen controller ProOx 360	Control oxygen level in large chamber and incubator
CO₂ controller ProCO₂	Control CO₂ level in chamber and incubator
CO₂ controller off-gas ProCO₂-PPM	Keep lower CO₂ level not to increase it
Oxygen/CO₂ controller C21	Control oxygen and CO₂ for C-chamber
Oxycycler C type	Programable controller for cell culture
Oxycycler A type	Programable controller for animal experiment
C-Chamber	Chamber for cell culture
Animal Chamber	Chamber for animal experiment

Application examples

Product information based on application examples.

Application examples for cell culture

Example 1	Create hypoxia or hyperoxya condition in incubator
Example 2	Create hypoxia or hyperoxya condition in a part of incubator. Reduce gas cunsumption and need more precise oxygen control.
Example 3	Oxygen variation with time
Example 4	Long term cell culture without exposing atmosphere

[Research examples]
Gene expression research of cancer cells
Test for sensitivity to drugs
Botanical experiment in hypoxia and higher oxygen condition.

Application examples for animal and botanical experiment

Example 5	Experiment for small animal(mouse and rats etc) in hypoxia or higher oxygen condition
Example 6	Oxygen variation with time for animal experiment
Example 7	Gas supply method without gas cylinder
Example 8	Remove CO₂, emitted by animals, from animal chamer
Example 9	Expose to high CO₂ condiion
Example 10	Botanical experiment in hypoxia and higher oxygen condition

[Research examples]
Experiment for mouse and rats with retinopathy of prematurity or pulmonary hypertension Research of Hypoxia Ischemia, Aleep Apnea Syndrome, Fraction of Inspiratory Oxygen etc.

There are various experiment applications for not only cell and animal but also bacteria and plant.

Application example 1 : [Cell Culture] Create hypoxia or hyperoxya condition in incubator.

Just add on oxygen controller ProOx110 to CO₂ incubator and controll oxygen level in it.

Because we can use existing incubator, we don't have to get new incubator or secure a place for it. If you want to control large incubator, we recommend to use oxgen controller ProOx 360.

Controller and peripheral equipment needed
Oxygen controller ProOx110 CO₂incubator, Gas cylinder and pressure regulator

Application example 2 : [Cell Culture] Create hypoxia or hyperoxya condition in a part of incubator. Reduce gas cunsumption and need more precise oxygen control.

When we use ProOx in conjunction with C-chamber, it can reduce gas consumption and cotrol oxygenelevel more precisely because the volume of chamber is smaller. Especially, if you want to create lower oxygen, less than 1%, we rcommend to use C-chamber, too.

Create hypoxia or hyperoxya condition in a part of incubator. Reduce gas cunsumption and need more precise oxygen control

It is possible to put multiple C-chamber in incubator and control them simultaneously. We can control CO₂ level by ProCO₂ as well. The control CO₂ range is 0.1～20.0%.

Controller and peripheral equipment needed
Oxygen controller ProOx 110, CO₂ controller ProCO₂, C-chamber, Incubator, Gas cylinder and pressure regulator

About C-Chamber

Combination C-chamber and ProOx and ProCO₂ lead to reduce the gas consumptions and more precise control.

C-chamber

Small value chamber lead to reduce the gas consumption
Durable and reduced maintenance
Using magnetic sealing for the front door
Include water tray

Dimentsion of C-chamber (WxDxH)

C-174 38.0 ｘ 30.9 x 14.7cm
C-274 38.0 ｘ 30.9 x 16.6cm
C-374 38.0 ｘ 30.9 x 21.7cm
C-474 38.0 ｘ 30.9 x 26.8cm

We can also use small chameber or box, should be semi-sealed, instead of C-chamber.

Application example 3 : [Cell Culture] Oxygen variation with time constantly

Oxycycler is programable controller, which create various oxygen level with time.

Ocycycler control up to two C-chamber simaltaneously by PC.

It can create various oxygen level with time.

Controller and peripheral equipment needed
Oxycycler C42, C-chamber Incubator, Gas cylinder and pressure regulator

Application example 4 : [Cell Culture] Long term cell culture without exposing atmosphere

When we have to take culture dishes from multi gas incubator to chage media and add regent to media during cell cultivation, cells will be exposed to atomosphere and environment around cell will be changed dramatically. We propose to use C-shuttle or Xvivo, if the environment around cells shold not be changed.

Manupilateion

Create same gas condition in C-shattle by controller. The cells is not exposed to atomosphere even during manupilation for cell culture experiment.

Transfer

It is easy to detach oxygen sensor and gas tube from C-chamber. And it keeps gas condition in C-chamber to atomoshere.

Culture

We can put C-chamber in incubator to cultivate cells in the chamber. Also ProOx and ProCO2 can control appropriate gas condition in chamber.

Application example 4 : [ Cell Culture] Long term cel culture without exposing atmosphere

Controller and peripheral equipment needed
C-shuttle, Oxygen controller ProOx110, CO₂ controller ProCO₂, C-chamber Incubator, Gas cylinder and pressure regulator

Xvivo Workstation

Xvivo workstation is cell incubation & processing system in which we can have carry out cell cultivation experiment in same condirion provided by Xvivo.

More detail information about Xvivo »

Application example 5 : [Animal and botanical experiment] Experiment for small animal(mouse and rats etc) in hypoxia or higher oxygen condition

With animal chamber, gas consumption would be reduced and it lead to more precise gas control.

Controller and peripheral equipment needed

Controller and peripheral equipment needed
Oxygen controller ProOx110 or 360/Animal chamber Gas cylinder and pressure regulator

Feature of animal chamber

Due to ventilation holes, it can weck moisture from them
Includes small fan to circurate gas in animal chamber
Easy to clean
Made by polypropylene

Size of animal chamber(cm)

Model	W	D	H
A-15274-P	38.1	50.8	50.8
A-30274-P	76.2	50.8	50.8
A-66274-P	167.6	50.8	50.8

Application example 6 : [Animal and botanical experiment] Oxygen variation with time for animal experiment

Following system is programable controller and system, which create various oxygen level with time.

Proposal 1 : Two oxygen controller with two gas timers

Combination of oxygen cotroller with gas timer provide oxegen variation with time.

Controller and peripheral equipment needed
Oxygen controller ProOx/Gas timer/Animal chamber Gas cylinder and pressure regulator

Proposal 2 : Using OxyCycler A84

OxyCycler A84, programable controller, can control up to four different condition.

Controller and peripheral equipment needed
OxyCycler A84/Animal chamber Gas cylinder and pressure regulator

Application example 7 : [Animal and botanical experiment] Gas supply method without gas cylinder

It’s troublesome to replace N2 gas cylinders

Nitrogen generator, which allow production of high-purity nitrogen from air, avoide the trouble of havint to exchang gas cylinders.

Application example 8 : [Animal and botanical experiment] Remove CO₂, emitted by animals, from animal chamer

ProCO₂-PPM is off-gas controller, which keeps CO₂ level (0~9999ppm) from rising in animal chamber. Once setting controller, it provides animal chamber with dry-air to keep CO₂ level lower than set point. It can be used with Oxygen controller ProOx.

I want to control CO2 level increases of small animal’s breathing in animal chamber.

Controller and peripheral equipment needed
Oxygen controller ProOx, CO₂ controller ProCO₂-PPM, Animal chamber Gas cylinder and pressure regulator

Application example 9 : [Animal and botanical experiment] Expose to high CO₂ condiion

ProCO₂ create higher CO₂ level (0.1~20.0%) in Animal chamber

I want to execute animal experiment in CO2 enriched condition.

Controller and peripheral equipment needed
CO₂controller ProCO₂, Animal chamber Gas cylinder and pressure regulator

Application example 10 : [Animal and botanical experiment] Botanical experiment in hypoxia and higher oxygen condition.

Oxygene controller may control gas condition in exsiting chamber.

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キーワード分類1	Cell culture
キーワード分類2	Enviroment control
キーワード分類3
製品カテゴリ1	Device
製品カテゴリ2	Cell culture
分野	Drug discovery\|Cancer\|Regenerative medicine\|Organ simulator\|Biological imaging\|Cell imaging
サムネイル画像
説明文	Limitless applications for your incubator, your protocols, your cell culture.
リダイレクトURL

キーワード分類1	Animal experiment
キーワード分類2	Enviroment control
キーワード分類3
製品カテゴリ1	Device
製品カテゴリ2	Animal experiment
分野	Drug discovery\|Cancer\|Regenerative medicine\|Cardiology\|Ophthalmology\|Pediatrics\|Pathological animal model
サムネイル画像
説明文	Limitless applications for your animal chamber
リダイレクトURL